Composite

Part:BBa_K510017

Designed by: David Caballero & Fernando Govantes   Group: iGEM11_UPO-Sevilla   (2011-09-19)
Revision as of 21:27, 19 September 2011 by Registry (Talk | contribs)

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pUC18R6KT-miniTn7BB-Gm-tetR

The mini-Tn7-Gm-tetR artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm (BBa_K510012) by inserting a tetR/ptetR cassette (BBa_I732083) in its BioBrick cloning site (BCS). This plasmid can be used as inducible expression system in single copy by integrating the device in the genome of the working organism.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4528
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4528
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4534
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4528
    Illegal BglII site found at 3212
    Illegal BglII site found at 3483
    Illegal BglII site found at 3769
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4528
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4528
    Illegal XbaI site found at 4543
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1854
    Illegal SapI site found at 518


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