Tag

Part:BBa_K638402

Designed by: Matthew Jones   Group: iGEM11_Cambridge   (2011-09-19)
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TorA tag variant

We found a TorA sequence which had been successfully used to export GFP to the periplasm of E.coli. The sequence was originally taken from this paper: Export of active green fluorescent protein to the periplasm by the twin-arginine translocase (Tat) pathway in Escherichia coli Joanna D. Thomas1, Richard A. Daniel2, Jeff Errington2, Colin Robinson1,*

The sequence was added into our constructs as part of a gibson assembly primer.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
n/aTorA tag variant