Part:BBa_K538310:Design

Cpn10/60 operon (pBAD)

Cpn10/60 K538310

This part is one of team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011]'s cold resistance BioBricks, also known as a CryoBrick. When transformed into E. coli, it should bestow a certain degree of cold resistance. It was placed under control of the strong arabinose-induced promoter derived from pBAD by team [http://2009.igem.org/Team:British_Columbia British Columbia 2009] (BBa_K206000). This means it will only be active in presence of arabinose, effectively turning the Brick off by default, and allowing it to be switched on by adding arabinose. When the arabinose is consumed, the Brick reverts to its off state. The promoter was taken from 2011's Spring Distribution Kit, as was the incorporated RBS. The coding regions of this Brick are new submissions to the registry, also submitted separately as BBa_K538000 (Cpn10) and BBa_K538001 (Cpn60). Functionally, this brick should be identical to BBa_K538311. Regardless, both bricks were created, so one could be used in case assembly of the other failed.

For more information about the subparts' purpose and design, refer to their main page or design page, respectively.

• BBa_K206000
  • Main Page
  • Design Page
• BBa_B0034
  • Main Page
  • Design Page
• BBa_K538000
  • Main Page
  • Design Page
• BBa_K538001
  • Main Page
  • Design Page

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 125
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1349
  Illegal BamHI site found at 65
  Illegal BamHI site found at 1415
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 208