Protein coding sequences/Biosynthesis/Bisphenol A

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Bisphenol A is a toxin that has been shown to leech from certain types of plastic. Studies have shown this chemical to have detrimental effects in animal studies and is very likely to be harmful to humans as well. The following bisphenol A degradation protein coding sequences are from Sphingomonas bisphenolicum AO1 and may aid in the remediation of bisphenol A contamination.

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Name	Description	Length	Status
BBa_K525515	Fusion protein of BisdA and BisdB	1602	In stock
BBa_K525517	Fusion protein of BisdA and BisdB behind constitutive promoter	1663	In stock
BBa_K123000	BisdA	330	It's complicated
BBa_K123001	BisdB	1284	It's complicated
BBa_K525007	Fusion protein of S-Layer SgsE with T7/lac and BisdA and BisdB	3999	Not in stock
BBa_K525008	Fusion protein of S-Layer SbpA with T7/lac and BisdA and BisdB	4791	Not in stock
BBa_K525582	Fusion protein of NADP+ Oxidoreductase and BisdA and BisdB, polycistronic	2448	Not in stock
BBa_K525562	Fusion protein of NADP+ Oxidoreductase and BisdA and BisdB with middle strong promoter,RBS	2410	Not in stock

Jason Gardiner and the [http://2008.igem.org/Team:The_University_of_Alberta 2008 University of Alberta iGEM team] designed a set of parts for degradation of bisphenol A.

In 2011, the iGEM team from [http://2011.igem.org/Team:Bielefeld-Germany Bielefeld University] could show that fusing BisdA and BisdB together to one protein leeds to an improvement of the bisphenol A degradation.

Background of bisphenol A degradation

Bisphenol A is mainly hydroxylated into the products 1,2-Bis(4-hydroxyphenyl)-2-propanol and 2,2-Bis(4-hydroxyphenyl)-1-propanol. In S. bisphenolicum AO1, a total of three genes are responsible for this BPA hydroxylation: a cytochrome P450 (CYP, bisdB), a ferredoxin (Fd, bisdA) and a ferredoxin-NAD⁺ oxidoreductase (FNR) Sasaki05a. The three gene products act together to reduce BPA while oxidizing NADH + H⁺. The cytochrome P450 (BisdB) reduces the BPA and is oxidized during this reaction. BisdB in its oxidized status is reduced by the ferredoxin (BisdA) so it can reduce BPA again. The oxidized BisdA is reduced by a ferredoxin-NAD⁺ oxidoreductase consuming NADH + H⁺ so the BPA degradation can continue Sasaki05a. This electron transport chain between the three enzymes involved in BPA degradation and the BioBricks needed to enable this reaction in vivo and in vitro are shown in the following figure (please have some patience, it's an animated .gif file):

Fig. 1: Animation of proposed reaction mechanism of bisphenol A hydroxylation by the involved enzymes FNR (BBa_K525499, Fd (BisdA, BBa_K123000) and CYP (BisdB, BBa_K123001)

References

Sasaki pmid=18492046
Zhang pmid=17291567
Nakajima pmid=12354921
Sasaki05a pmid=16332782
Sasaki05b pmid=15865158
Kang02 pmid=12202920
Kang04 pmid=15242458

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