Generator

Part:BBa_K584026

Designed by: Bakul Jitendra Vinchhi, Sarah Veugelen, Katrien Vandermeeren, Yana Hoorne   Group: iGEM11_KULeuven   (2011-09-19)
Revision as of 06:56, 19 September 2011 by Vinchhi (Talk | contribs)

Constitutive_Promoter + AFP(Antifreeze extra-cellular Protein Generator)

This part contains the constitutive promoter (J23119) and the Antifreeze Protein (AFP) sequence behind it.

The part BBa_K584019 contains [ OmpA_transmembrane_domain + Linker + point_mutant_HPLC6(AFP) ] with the B0034 RBS and B0015 Terminator.

A point mutation at Base Pair number 156 of the HPLC6 sequence was performed by replacing the T in the wild-type coding sequence to C. This mutation was necessary as the sequence CTGCAG in the Wild-type coding sequence corresponded to a Pst1 restriction site. GCT(Wild type) and GCC(1 point mutant) are both valid triplet codons for Alanine.

Detailed information of the Pseudopleuronectes americanus Antifreeze Protein (HPLC gene) can be found on this NCBI [http://www.ncbi.nlm.nih.gov/nuccore/M62417 webpage].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None