Composite

Part:BBa_K611012

Designed by: Nicholas Csicsery   Group: iGEM11_UC_Davis   (2011-09-18)
Revision as of 00:14, 19 September 2011 by Registry (Talk | contribs)

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pTet Characterization Construct

This part is used to characterize mutants of BBa_R0040 (pTet). By adding a mutant of BBa_R0040 before this part, one can measure several of its properties through the expression of GFP. Certain characteristics of the mutant, such as the binding of the TetR repressor, can be measured after inducing the pBad promoter with arabinose. Concentration of the repressor can be varied through different arabinose concentrations as long as AraC is present.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1008
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 948
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 662


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Parameters
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