Measurement

Part:BBa_K528012

Designed by: Elżbieta Jankowska   Group: iGEM11_Warsaw   (2011-09-18)
Revision as of 22:02, 18 September 2011 by Pawel29 (Talk | contribs) (→‎Measurements)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

RBS measurement device J23100 B0032 Super Folder GFP

This device was used to determine BBa_B0032 strength relative to BBa_B0034.

Construct design

The device consists of BBa_K299502 expression vector J23100+B0032. BBa_I746916 SF-GFP was used as reporter protein.

Authors

Clonning:

  • Paweł Urbański
  • Elżbieta Jankowska
  • Krzysztof Szczepaniak
  • Dorota Kaja Sabat

The lab work was supervised by:

  • Michał Lower
  • Ania Olchowik

Measurements

Using this construct we have determined that strength of BBa_B0032 with SF-GFP is 94,31% relative to BBa_B0034 with SF-GFP.

All our SF-GFP measurement results are summarized on the chart below:

Wyk8.png

For more info please look at Team Warsaw 2011 [http://2011.igem.org/Team:Warsaw/RBSmeasurement].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 75


[edit]
Categories
Parameters
None