Measurement

Part:BBa_K528003

Designed by: Dorota Kaja Sabat   Group: iGEM11_Warsaw   (2011-09-18)
Revision as of 21:51, 18 September 2011 by Pawel29 (Talk | contribs) (→‎Measurements)

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RBS measurement device J23100 B0031 RFP

This device was used to determine BBa_B0031 strength relative to BBa_B0034.

Construct design

The device consists of BBa_K299501 expression vector J23100+B0031. E1010 RFP was used as reporter protein.

Authors

Clonning:

  • Paweł Urbański
  • Elżbieta Jankowska
  • Krzysztof Szczepaniak
  • Dorota Kaja Sabat

The lab work was supervised by:

  • Michał Lower
  • Ania Olchowik

Measurements

Using this construct we have determined that strength of BBa_B0031 with RFP is 8,29% relative to BBa_B0034 with RFP.

All our RFP measurement results are summarized on the chart below:

Wyk6.png

For more info please look at Team Warsaw 2011 [http://2011.igem.org/Team:Warsaw/RBSmeasurement].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 618
    Illegal AgeI site found at 730
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None