![](https://parts.igem.org/images/partbypart/icon_plasmid.png)
Plasmid
Part:BBa_K510013:Design
Designed by: David Caballero Group: iGEM11_UPO-Sevilla (2011-09-18)
pUC18R6KT-miniTn7BB-Cm
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 4640
Illegal suffix found in sequence at 1 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4640
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 4646 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4640
Illegal BglII site found at 3212
Illegal BglII site found at 3483
Illegal XhoI site found at 3569 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 4640
Illegal suffix found in sequence at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 4640
Illegal XbaI site found at 4655
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1854
Illegal SapI site found at 518
Design Notes
NcoI and SphI sites were added at the ends of the chloramphenicol resistance cassette to facilitate cloning
Source
The pUC18R6KT-mini-Tn7-Cm is a derivative of pUC18R6KT-mini-Tn7-Gm (BBa_K510012) by replacing the gentamycin resistance cassette by a chloramphenicol resistance cassette amplified from pSB1C3 with these primers: tatGCATGCCCATGGaacttggtctgacagctcgag and tatGCATGCCCATGGtcgggcacgtaagaggttcc.
References
Kyoung-Hee Choi, Jared B Gaynor, Kimberly G White, Carolina Lopez, Catharine M Bosio, RoxAnn R Karkhoff-Schweizer & Herbert P Schweizer (2005). A Tn7-based broad-range bacterial cloning and expression system. Nature Methods, vol.2 NO.6, June 2005, 443.