Plasmid

Part:BBa_K510014:Design

Designed by: David Caballero   Group: iGEM11_UPO-Sevilla   (2011-09-18)
Revision as of 19:47, 18 September 2011 by Dcabpra (Talk | contribs) (→‎Design Notes)

pUC18R6KT-miniTn7BB-Km


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4799
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4799
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4805
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4799
    Illegal BglII site found at 3212
    Illegal BglII site found at 3483
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4799
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4799
    Illegal XbaI site found at 4814
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1854
    Illegal SapI site found at 518


Design Notes

Source

The pUC18R6KT-mini-Tn7-Km is a derivative of pUC18R6KT-mini-Tn7-Gm (BBa_K510012) by replacing the gentamycin resistance cassette by a kanamycin resistance cassette amplified from pSB4K5 with these primers: tatGCATGCCCATGGattggggctcactcaaagg and tatGCATGCCCATGGtcgacaatgtaactactag.

References