Composite
Part:BBa_K546005
Designed by: Matthijn Hesselman Group: iGEM11_Wageningen_UR (2011-09-18)
Lux pL controlled LuxR + lux pR autoinducing LuxI (lva tag) + lux pR controlled GFP(lva tag).
in progress
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3909
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1101
Illegal BsaI.rc site found at 1828
Illegal BsaI.rc site found at 2086
Illegal BsaI.rc site found at 3189
[edit]
Categories
Parameters
//classic/signalling/receiver
//function/biosynthesis/ahl
//function/cellsignalling
//function/biosynthesis/ahl
//function/cellsignalling
control | (1) R0063 & (2) R0062 & (3) R0062 |
device_type | feedback |
direction | Forward |
latency | Seconds |
protein | (1) LuxR _ (2) GFP-LVA (3) luxI-LVA |
signalling_molecule | 3OC6HSL (AHL) |
switch_point | (1) 2nM |