Tag

Part:BBa_K608408

Designed by: Rüdiger Trojok   Group: iGEM11_Freiburg   (2011-09-14)
Revision as of 18:37, 18 September 2011 by Ruediger (Talk | contribs)

GST tag

The GST-tag has the size of 220 amino acids (roughly 26 KDa). It is fused to the N-terminus of the precipitator protein BBa_K608406 and includes a PreScission Protease domain for cleavage of the GST tag during protein purification.

http://www.gelifesciences.com/aptrix/upp01077.nsf/content/Products?OpenDocument&parentid=976040&moduleid=38868&zone=Proteomics

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


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