Translational_Unit
RBS-GFP-T7

Part:BBa_K606029

Designed by: Axel Séguret, Cyrille Pauthenier   Group: iGEM11_Paris_Bettencourt   (2011-09-18)
Revision as of 18:00, 18 September 2011 by AxelS (Talk | contribs)

RBS SpoVG GFPmut3b T7terminator

This is a GFP expression system for B. Subtilis that is dont to be polymerazed by the T7 polymerase. This have to be activated by the pT7 promoter I712074. The RBS used is SpoVG, that is design for a strong expression in B. Subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 886
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 662


[edit]
Categories
//classic/reporter
Parameters
None