Composite

Part:BBa_K537014:Design

Designed by: Ezio Fok   Group: iGEM11_WITS-CSIR_SA   (2011-09-16)
Revision as of 16:45, 18 September 2011 by Sashrez (Talk | contribs) (→‎References)

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Promoter-Atrazine riboswitch-mCherry-Double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 52
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 683
    Illegal AgeI site found at 795
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Coming soon


Source

Atrazine Riboswitch-mRFP - BBa_K537008

Strong promoter – Bba_J23119

Double terminator – Bba_B0015

References

Sinha J., Reyes S.J., and Gallivan J.P. Reprogramming Bacteria to Seek and Destroy a Herbicide. 2010, Nat Chem Biol. 6(6): 464–470