Composite

Part:BBa_K537011:Design

Designed by: Ezio Fok   Group: iGEM11_WITS-CSIR_SA   (2011-09-16)
Revision as of 16:35, 18 September 2011 by Sashrez (Talk | contribs) (→‎Source)

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Promoter-Theophylline riboswitch 1-CheZ-Venus-Double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1360
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Coming soon


Source

Theophylline Riboswitch (type 1) + CheZ - BBa_K537001

Venus C-Fusion - BBa_K537006

Strong promoter – Bba_J23119

Double terminator – Bba_B0015

References

Lynch S.A. Desai S.K.,Sajja,H.K., and Gallivan J.P.A high-trhoughput screen for synthetic riboswitches reveals mechanistic insight into their function. 2007, Chem Biol 14:173-184

Topp S. and Gallivan J.P. Random walks to synthetic riboswitches – a high throughput selection based on cell motility. 2008, ChemBiochem 9:210-213