Composite

Part:BBa_K515104

Designed by: Atipat Patharagulpong   Group: iGEM11_Imperial_College_London   (2011-09-07)
Revision as of 14:35, 18 September 2011 by Cs3109 (Talk | contribs)

J23100 promoter - Antiholin

A composite part between J23100 and Anti-holin (BBa_K515004). The promoter has been chosen in order to comply with our Gene Guard specifications. It is under the influence of a strong promoter due to the lower copy number of this gene (will be integrated into the genome) in comparison to that of the Holin gene which will be in a high copy number plasmid within our system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 276


[edit]
Categories
//biosafety/kill_switch
Parameters
chassisE. coli DH5α
controlJ23100
device_typeHolin repressing protein
proteinBBa_K515004
resistanceChloramphenicol