Composite

Part:BBa_K553005:Design

Designed by: Giulio Bernardinelli, Veronica Parisi, Francesca Cesaratto   Group: iGEM11_UNITS_Trieste   (2011-09-17)
Revision as of 14:29, 18 September 2011 by Giulio (Talk | contribs) (→‎Design Notes)

C. firmi beta-glucosidase PTraI regulated


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
    Illegal XhoI site found at 1251
    Illegal XhoI site found at 1484
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 740
    Illegal AgeI site found at 183
    Illegal AgeI site found at 242
    Illegal AgeI site found at 636
    Illegal AgeI site found at 831
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 613


Design Notes

Assembling of a new biobrick BBa_K553002 (obtained by pcr from A.Tumefaciens gDNA), the RBS-glucosidase complex (BBa_J15001 + BBa_K392008 a gift from Dr. C. French, Edinbourg Univ.) and the double terminator BBa_B0015.

Source

uhuip

References