Part:BBa_K515104

J23100 promoter - Antiholin

A composite part between J23100 and Anti-holin <a href="https://parts.igem.org/Part:BBa_K515004:Design">(BBa_K515004)</a>. The promoter has been chosen in order to comply with our Gene Guard specifications. It is under the influence of a strong promoter due to the lower copy number of this protein (will be integrated into the genome) in comparison to that of the Holin which will be in a high copy number plasmid within our system.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 276