Part:BBa_K527002

Biosynthesis/Oximes/CYP79B1/Plug 'n' Play

This BioBrick BBa_K527002 encode the plant enzyme Cytochrome P450 CYP79B1. Cytochromes P450 are defined as heme-thiolate proteins featuring a particular spectral signature at 450 nm, thus the name. Despite the label as cytochromes, these proteins are not involved in electron transfers, but act as monooxygenases in a wide range of reactions such as epoxidation, N-dealkylation, O-dealkylation, S-oxidation and hydroxylation.

CYP79B1 is an N-hydroxylase converting L-Tryptophan into indole 3-acetaldoxime, the precursor of a cyanogenic glycoside . Oximes as the indole 3-acetaldoxime is generally toxic to fungi and is frequently used as a chemical fungicide. They function by inhibiting mitochondrial oxidases and thereby promote lipid peroxidation and the production of toxic reactive oxygen species.

BBa_K527002 is submitted in the plasmid BBa_K678023 due to the different assembly method (Plug 'n' Play) hence the missing prefix and suffix.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

• Naur P, Hansen P H, Bak S, Hansen B G, Jensen N B, Nielsen H L, Halkier B A (2002). CYP79B1 from Sinapis alba converts tryptophan to indole-3-acetaldoxime. Archives of Biochemistry and Biophysics Vol. 409, 235–241.

• Møller B L (2010). Dynamic Metabolons. Science Vol. 330, 1328-1329.