Measurement

Part:BBa_K528000

Designed by: Pawel Urbanski   Group: iGEM11_Warsaw   (2011-09-18)
Revision as of 14:07, 18 September 2011 by Pawel29 (Talk | contribs)

RBS measurement device J23100 B0031 mORANGE

This device was used to determine BBa_B0031 strength relative to BBa_B0034.

Construct design

The device consists of BBa_K299501 expression vector J23100+B0031. E2050 mORANGE was used as reporter protein.

Authors

Clonning:

  • Paweł Urbański
  • Elżbieta Jankowska
  • Krzysztof Szczepaniak
  • Dorota Kaja Sabat

The lab work was supervised by:

  • Michał Lower
  • Ania Olchowik

Flow cytometer measurements done by Cherry Moreno

Measurements

Using this construct we have determined that BBa_B0031 strength is 69,73% relative to BBa_B0034

<img src="Wyk5.png" alt="ERROR" />


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 826
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None