Reporter
mRFP1 C-fu

Part:BBa_K537005:Design

Designed by: Gloria Hlongwane   Group: iGEM11_WITS-CSIR_SA   (2011-09-09)
Revision as of 13:52, 18 September 2011 by Sashrez (Talk | contribs) (→‎Design Notes)

mCherry (mRFP1) C-Fusion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 552
    Illegal AgeI site found at 664
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This BioBrick represents a C-terminal fusion part. The part contains a standard suffix and a “assembly standard 25” prefix (Freiburg Fusions/ RFC 25). This version of the mRFP reporter (BBa_K354002) has the standard BioBrick suffix and prefix. The standard Biobrick prefix, however, is followed by another restriction site – specific for NgoMIV (which forms the Freiburg fusion prefix). The part lacks an ATG start codon (provided by N-terminal part). It can therefore be fused to any gene in a modular fashion via the modular construction strategy of protein fusion developed by the Freiburg 2007 team.

Source

The mRFP1 fluorescent reporter is derived from part BBa_E1010

References