Coding
ChZ N-fusi

Part:BBa_K537007:Design

Designed by: Ezio Fok   Group: iGEM11_WITS-CSIR_SA   (2011-09-12)
Revision as of 13:46, 18 September 2011 by Sashrez (Talk | contribs) (→‎Design Notes)

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RBS CheZ N-Fusion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biobrick part represents a composite of a RBS directly upstream of an E.coli CheZ motility factor.

This part represents an N-terminal fusion part. The part contains a standard prefix and a “assembly standard 25” suffix (Freiburg Fusions/ RFC 25). Because of multiple AgeI sites within CheZ, full compatibility with RFC 25 could is not maintained. Cloning to a C-terminal part occurs by cutting with NgoMI. The CheZ CDS therefore lacks a TAATAA double stop codon (TAG stop provided by RFC 25 suffix).

Source

The CheZ sequence was PCR amplified from E.coli strain DH5α.

References