Generator
atRS-RFP

Part:BBa_K537008

Designed by: Alexandra Reznichenko   Group: iGEM11_WITS-CSIR_SA   (2011-09-12)
Revision as of 13:35, 18 September 2011 by Sashrez (Talk | contribs)

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Atrazine Riboswitch-mRFP

This part consists of an atrazine-sensitive riboswitch, developed by Sinha et al (2010), detached from its associated translation unit (coding region) and fused to mRFP1 fluorescent reporter (BBa_J61100). This part regulates the expression of mRFP in an atrazine-dependent fashion through an atrazine-specific RNA aptamer.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 9
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 640
    Illegal AgeI site found at 752
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None