Coding

Part:BBa_K527001:Design

Designed by: Eva Meier Carlsen   Group: iGEM11_Copenhagen   (2011-09-17)
Revision as of 13:34, 18 September 2011 by EvaCar (Talk | contribs) (→‎Source)

Biosynthesis/Oximes/CYP79A2/Plug 'n' Play


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 165
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 432


Design Notes

Mutated at a Pst1 restiction site (nucleotide 342) No prefix and suffix. User assembly ends.


Source

Institute of Plant Biology and Biotechnology

Faculty of LIFE Science

University of Copenhagen

Denmark

http://2011.igem.org/Team:DTU-Denmark-2

References

  • Wittstock U and Halkier B A (2000). Cytochrome P450 CYP79A2 from Arabidopsis thaliana L. Catalyzes

the Conversion of L-Phenylalanine to Phenylacetaldoxime in the Biosynthesis of Benzylglucosinolate. The Journal of Biological Chemistry Vol. 275, No. 19, Issue of May 12, pp. 14659–14666.

  • Møller B L (2010). Dynamic Metabolons. Science Vol. 330, 1328-1329.