Generator
atRS-ChZ

Part:BBa_K537000:Design

Designed by: Natasia Kruger   Group: iGEM11_WITS-CSIR_SA   (2011-07-19)
Revision as of 13:28, 18 September 2011 by Sashrez (Talk | contribs) (→‎Source)

Atrazine Riboswitch-CheZ fusion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 9
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The effectiveness of the atrazine riboswitch is determined by the ability of the RNA 'loop' to hide the RBS preventing translation. The sequence of bases within the riboswitch will determine the formation of the loop and its leakiness.

Source

The CheZ gene was isolated from genomic DNA using PCR where the forward primers in the reaction contained the sequence for the atrazine riboswitch.

The mRFP1 fluorescent reporter is derived from part BBa_E1010

References

Sinha J., Reyes S.J., and Gallivan J.P. Reprogramming Bacteria to Seek and Destroy a Herbicide. 2010, Nat Chem Biol. 6(6): 464–470