Generator
thRS2-V

Part:BBa_K537004:Design

Designed by: Gloria Hlongwane   Group: iGEM11_WITS-CSIR_SA   (2011-09-09)
Revision as of 13:22, 18 September 2011 by Sashrez (Talk | contribs) (→‎References)

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Theophylline Riboswitch 2-Venus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 687
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biobrick part represents a composite of a theophylline riboswitch (type2/clone 12.1) upstream of a Venus fluorescent protein reporter (derived from part BBa_K354002).

Previous teams: Lethbridge 2007, Lethbridge 2009, and NYMU Taipei 2010 all considered strategies for making the riboswitch a separate biobrick, however standard assembly techniques are inadequate since the sequence distance between the ATG, RBS and aptamer domain of the riboswitch are critical. We have explored this further in a more elaborate discussion here.

Source

The Venus fluorescent protein is derived from BBa_K354002.

References

1. Lynch S.A. and Gallivan J.P., A flow cytometry based screen for synthetic riboswitches. 2009, Nucleic Acids Res. 37(1)184-192

2. Topp S, Gallivan JP. Guiding bacteria with small molecules and RNA. J Am Chem Soc 2007;129:6807-11