Generator
thRS1-V

Part:BBa_K537003:Design

Designed by: Gloria Hlongwane   Group: iGEM11_WITS-CSIR_SA   (2011-09-09)
Revision as of 13:18, 18 September 2011 by Sashrez (Talk | contribs) (→‎References)

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Theophylline Riboswitch 1-Venus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 686
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biobrick part represents a composite of a theophylline riboswitch (type1/clone 8.1) upstream of a Venus fluorescent protein reporter (derived from part BBa_K354002).

Previous teams: Lethbridge 2007, Lethbridge 2009, and NYMU Taipei 2010 all considered strategies for making the riboswitch a separate biobrick, however standard assembly techniques are inadequate since the sequence distance between the ATG, RBS and aptamer domain of the riboswitch are critical. We have explored this further and that is why we decided to synthesise this part via PCR. This part therefore represents a fused part, rather than a composite part that is made out of single biobricks.

Source

Topp and Gallivan JACS 2007 (and also BBa_K249026 and BBa_K411001) fusion with Venus reporter BBa_K354002.

References

1. Topp S, Gallivan JP. Guiding bacteria with small molecules and RNA. J Am Chem Soc 2007;129:6807-11

2. Lynch S.A. Desai S.K.,Sajja,H.K., and Gallivan J.P.A high-trhoughput screen for synthetic riboswitches reveals mechanistic insight into their function. 2007, Chem Biol 14:173-184

3. Topp S. and Gallivan J.P. Random walks to synthetic riboswitches – a high throughput selection based on cell motility. 2008, ChemBiochem 9:210-213