Device
Part:BBa_K546546:Design
Designed by: M. Hesselman and B. Ryback Group: iGEM11_Wageningen_UR (2011-09-14)
The Synchroscillator: A Tunable Synchronized Oscillatory System
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2781
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1184
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 985
Illegal BsaI.rc site found at 2042
Illegal BsaI.rc site found at 2930
Illegal BsaI.rc site found at 3657
Design Notes
Some parameters of the signalling molecule formation and degradation were unknown. Modeling of the BioBrick device was therefore hard. The hybrid promoters were installed to stablize the oscillation in case it would be necessary.
Source
The lux pR promoter originates from the quorum sensing ability of Vibrio fischeri.