Coding

Part:BBa_K572005:Design

Designed by: Avaneesh T   Group: iGEM11_IIT_Madras   (2011-09-18)
Revision as of 07:43, 18 September 2011 by Registry (Talk | contribs)

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Proteorhodopsin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene had E,S and P sites. Thus, we used site directed mutagenesis to optimize the codon and to remove the E,S and P sites so that it can be ligated with the iGEM plasmid backbone.


Source

Source Organism : Marine gamma proteobacterium EBAC31A08 We would like to thank Kwang-Hwan "Kevin" Jung, Ph.D., Associate Professor, Department of Life Science and Institute of Biological Interfaces, Sogang University, Korea for sending us the plasmid (pKJ900) with proteorhodopsin gene.

References