Not Released
Sample In stock
Experience: Works
Not Used
Get This Part
Composite

Part:BBa_K539281

Designed by: Yang, Yi-Pei   Group: iGEM11_NCTU_Formosa   (2011-09-17)
Revision as of 04:32, 18 September 2011 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


rbs+crtZ(beta-carotene hydroxylase) under heat sensitive cI regulater

This part contains crtZ, which works as the zeaxanthin biosynthesis pathway when add beta-carotene as a substrate. crtZ is regulated by cI and cI regulated promoter. The promoter has two DNA binding sites for lambda cI repressor BBa_C0051. cI binding results in repression of transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1492
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None