DNA

Part:BBa_K676009:Design

Designed by: Kheng Tee Ng   Group: iGEM11_UCL_London   (2011-09-17)
Revision as of 18:00, 17 September 2011 by Registry (Talk | contribs)

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Gyrase B


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 428
    Illegal BglII site found at 1667
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 100
    Illegal AgeI site found at 238
    Illegal AgeI site found at 931
    Illegal AgeI site found at 2047
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The ORF has a EcoR1 restriction site on the 6th bp position.


Source

The ORF comes from E.coli.

References