DNA

Part:BBa_K676007:Design

Designed by: Kheng Tee Ng   Group: iGEM11_UCL_London   (2011-09-17)
Revision as of 17:49, 17 September 2011 by Registry (Talk | contribs)

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Gyrase A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1593
    Illegal BglII site found at 2538
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 27
    Illegal AgeI site found at 703
    Illegal AgeI site found at 760
    Illegal AgeI site found at 1399
    Illegal AgeI site found at 2155
    Illegal AgeI site found at 2523
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 43
    Illegal SapI.rc site found at 51
    Illegal SapI.rc site found at 1510


Design Notes

This is the E.coli GyrA ORF with a Pst 1 restriction site at the 1212th bp position. SIte directed mutagenesis should be done to remove the site.


Source

E.coli genome

References