Coding
YF1
Part:BBa_K592004
Designed by: Lei Sun Group: iGEM11_Uppsala-Sweden (2011-09-17)
YF1 blue-light sensor protein
YF1 is a fusion protein of YtvA (B.subtilis) and FixL(Br.japonicum). It contains the N-terminal region of YtvA (1-127) and C-terminal region of FixL (258-505), interconnected by a helical linker. This fusion protein is designed by Andreas Moglich and Keith Moffat. As shown in previous research, Illumination of the fusion kinase YF1 reduced net kinase activity by ∼1000-fold in vitro. YF1 also controls gene expression in a light-dependent manner in vivo.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 544
Illegal NgoMIV site found at 616
Illegal NgoMIV site found at 706
Illegal NgoMIV site found at 724
Illegal AgeI site found at 258 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 157
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Categories
Parameters
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