Coding
YF1

Part:BBa_K592004

Designed by: Lei Sun   Group: iGEM11_Uppsala-Sweden   (2011-09-17)
Revision as of 14:13, 17 September 2011 by Registry (Talk | contribs)

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YF1 blue-light sensor protein

YF1 is a fusion protein of YtvA (B.subtilis) and FixL(Br.japonicum). It contains the N-terminal region of YtvA (1-127) and C-terminal region of FixL (258-505), interconnected by a helical linker. This fusion protein is designed by Andreas Moglich and Keith Moffat. As shown in previous research, Illumination of the fusion kinase YF1 reduced net kinase activity by ∼1000-fold in vitro. YF1 also controls gene expression in a light-dependent manner in vivo.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 544
    Illegal NgoMIV site found at 616
    Illegal NgoMIV site found at 706
    Illegal NgoMIV site found at 724
    Illegal AgeI site found at 258
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 157


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Categories
Parameters
None