Plasmid
Part:BBa_K510000:Design
Designed by: David Caballero, Fernando Govantes Group: iGEM11_UPO-Sevilla (2011-09-16)
Revision as of 11:38, 17 September 2011 by Fgovrom (Talk | contribs) (New page: The miniTn7BB-Gm synthetic minitransposon was digested at the flanking SfiI sites and cloned into SfiI-digested pUC18-Sfi. This results in the elimination of the complete pUC18-SfiI multi-...)
The miniTn7BB-Gm synthetic minitransposon was digested at the flanking SfiI sites and cloned into SfiI-digested pUC18-Sfi. This results in the elimination of the complete pUC18-SfiI multi-cloning site, except for the duplicated SfiI sites that remain on both sides of the transposon, thus facilitating its transfer to other vectors.