Part:BBa_K510000
pUC18Sfi-miniTn7BB-Gm
pUC18Sfi-miniTn7BB-Gm is a vehicle vector for the minitransposon miniTn7BB-Gm. This transposon is a part of the Tn7 BioBrick toolkit set that uses Tn7 derivatives for integration of BioBricks in single copy at a conserved target (the attTn7 site), in multiple bacteria. The structure of the mini-Tn7BB-Gm transposon is shown in the figure below. Tn7R and Tn7L are the right and left ends of Tn7 transposon, respectively, required for recognition of the transposase machinery and transposition. FRT is the Flp recombinase target site for excision of the resistance once the transposon is inserted in the genome of a living organism. The gentamycin resistance cassette (Gm) is flanked by restriction sites in order to facilitate the generation of variants with different antibiotic resistance markers. The BioBrick cloning site (BCS) includes the prefix and suffix restrictions sites used in assembly standard 10, and it is the place where BioBricks are inserted for genome integration. This plasmid can be used to maintain the mini-Tn7-Gm in E. coli and other enterics and may be used for transposition into the genomes of non-enteric bacteria, in which it is non-replicative. As a BioBrick cloning vector, pUC18Sfi-miniTn7BB-Gm is fully compatible with Assembly Standard 10
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 4367
Illegal suffix found in sequence at 1 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4367
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 4373 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4367
Illegal BglII site found at 3051
Illegal BglII site found at 3322
Illegal BglII site found at 3608 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 4367
Illegal suffix found in sequence at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 4367
Illegal XbaI site found at 4382
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1681
Illegal SapI.rc site found at 2763
//dna/transposon
//plasmid/chromosomalintegration
n/a | pUC18Sfi-miniTn7BB-Gm |