Composite
1C2

Part:BBa_K590054

Designed by: Lei Zheng, Ben Mo, Elaine Lai, Juhye An   Group: iGEM11_Washington   (2011-09-15)
Revision as of 18:45, 16 September 2011 by Anjuhye (Talk | contribs)

RrD_N25M-PSB1C3-High constitutive

This part consists of Acyl-ACP Reductase, ribosomal binding site, mutant Aldehyde Decarbonylase under the control of high consititutive promoter for alkane production.

Usage and Biology

Data collected from In Vivo Assay developed by University of Washington iGEM Team

When AAR and ADC are co-expressed, they produce odd chain length alkanes from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team].

GC plot showing that alkanes are only produced when both ADC and AAR are expressed.
GC-MS quantification shows greater alkane production under UW iGEM 2011's "optimized conditions" (see wiki for details)



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None