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Coding
Salty_PduJ

Part:BBa_K562004:Design

Designed by: Frank Sargent   Group: iGEM11_Dundee   (2011-09-16)
Revision as of 12:50, 16 September 2011 by Franksargent (Talk | contribs) (→‎Design Notes)

Salty_PduJK BMC Components


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 666
    Illegal BamHI site found at 805
    Illegal BamHI site found at 899
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 293
    Illegal AgeI site found at 560
    Illegal AgeI site found at 596
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The fragment carries an engineered BamHI site at its extreme 3' end, but note that this is because a generic primer design system was used. Be aware that the pduK gene carries 2 native BamHI sites and that translation is predicted to initiate at a GUG codon.

Source

Derived from Salmonella enterica serovar Typhimurium LT2 genomic sequence.

References