Coding
Salty_PduA

Part:BBa_K562009:Design

Designed by: Frank Sargent   Group: iGEM11_Dundee   (2011-09-16)
Revision as of 12:48, 16 September 2011 by Franksargent (Talk | contribs) (→‎Design Notes)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Salty_PduABTUNJK BMC Components


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1898
    Illegal BamHI site found at 3058
    Illegal BamHI site found at 3197
    Illegal BamHI site found at 3291
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1441
    Illegal NgoMIV site found at 2685
    Illegal AgeI site found at 1426
    Illegal AgeI site found at 2040
    Illegal AgeI site found at 2952
    Illegal AgeI site found at 2988
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Native PstI sites have been removed from pduB and pduU. There is an engineered BamHI site at the extreme 3' end, but please note that pduK contains two native BamHI sites that have not been removed. Be aware that pduK initiates at a GUG codon.

Source

Derived from Salmonella enterica serovar Typhimurium LT2 genomic sequence.

References