Part:BBa_K538003

CspA (Polaribacter irgensii)

Figure 1: Freeze/thaw cycle survival rate of E. coli containing an empty vector (black circles), a vector carrying P. irgensii 's CspA gene (white circles), and a vector carrying P. irgensii 's CspC gene (white triangles). Adapted from Uh et al. (2010)

This BioBrick encodes the CspA protein of the Arctic cryophile Polaribacter irgensii. It's homologous to E. coli 's cold-induced RNA chaperones CspA and CspD, and highly similar to P. irgensii's CspC (K538004). Uh et al. managed to increase the freeze/thaw cycle survival rate of E. coli more than five-fold by overexpressing this protein. (Figure 1)

Characterisation

[Update pending]

Safety

There are risks involved with enhancing bacterial cold resistance and facilitating their growth at low temperatures. Please refer to the [http://2011.igem.org/Team:Amsterdam/Project/Safety safety page] of this brick's developers.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]