Reporter
Part:BBa_K660000
Designed by: Glasgow Uni 2011 Group: iGEM11_Glasgow (2011-09-16)
LOV2 Domain
The LOV (Light-Oxygen-Voltage) domain is a photoreceptor that responds to blue light. In nature it was first found to be involved in the phototropism response in plants and has since been found to be present in fungi and bacteria also. It has been shown to be coupled to many domains, for example phosphodiesterase or kinases.
We are using it as a reporter due to its ability to function in anoxic conditions. This is particularly useful in biofilms and is a function that fluorescent proteins derived from GFP do not have.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 312
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 312
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 9
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 312
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 312
Illegal AgeI site found at 287 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 32
[edit]
Categories
Parameters
None |