Coding
AAR

Part:BBa_K590032

Designed by: Lei Zheng   Group: iGEM11_Washington   (2011-09-15)
Revision as of 02:35, 16 September 2011 by Swanson (Talk | contribs)

Acyl-ACP Reductase (AAR) - Synechococcus elongatus PCC7942

This part encodes an enzyme that reduces cellular fatty acyl-ACPs, from the bacterial fatty acid biosynthesis pathway, into fatty aldehydes. Use together with Aldehyde Decarbonylase (ADC) to generate alkanes from acyl-ACPs. This part has been codon-optimized for E. coli.

Fatty Acid Biosynthesis.jpg

Usage and Biology

The [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team] worked out conditions to grow E. coli containing AAR and ADC to generate alkanes.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1060
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None