Coding
mamHI

Part:BBa_K590000:Design

Designed by: Michael Brasino, Alicia Wong, Rashmi Ravichandran   Group: iGEM11_Washington   (2011-09-15)
Revision as of 02:08, 16 September 2011 by Saushun (Talk | contribs) (→‎References)

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mamHI, from Magnetospirillum magneticum


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 89
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 238
    Illegal NgoMIV site found at 1254
    Illegal NgoMIV site found at 2589
    Illegal NgoMIV site found at 3920
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 651
    Illegal BsaI site found at 1986
    Illegal BsaI site found at 3317
    Illegal SapI site found at 825
    Illegal SapI site found at 2160
    Illegal SapI site found at 3491


Design Notes

sequence confirmed


Source

Extracted from host genome Magnetospirillum magneticum strain AMB-1

References

D. Murat Quinlan, H. Vali, and A. Komeili. A Comprehensive genetic dissection of the magnetosome gene island reveals the step-wise assembly of a prokaryotic organelle. PNAS. 2010,  vol. 107, 12.