Composite

Part:BBa_K523022

Designed by: Mun Ching Lee, Sylvia Ispasanie   Group: iGEM11_Edinburgh   (2011-09-15)
Revision as of 20:45, 15 September 2011 by Allancrossman (Talk | contribs)

PlacLacZ + crtEIB

Lac promoter driving expression of crtE, crtI, and crtB (carotenoid synthesis pathway).

Part was assembled as a demonstration of Edinburgh's 2011 BioSandwich protocol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1651
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3665
    Illegal NgoMIV site found at 3795
    Illegal AgeI site found at 1346
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None