Signalling
Part:BBa_K546003
Designed by: Brendan Ryback, Matthijn Hesselman Group: iGEM11_Wageningen_UR (2011-09-14)
Lux pL controlled LuxR + lux pR promoter.
This part was made as a replacement for a defective version of BBa_F2621 which lacked the R0063 promoter, but was otherwise intact and functional. It shows a significantly higher response to 3OC6HSL than the incomplete BBa_F2621 (which is present in the Registry as BBa_K317037).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1101
[edit]
Categories
Parameters
//classic/signalling/receiver
//function/cellsignalling
//function/cellsignalling
control | R0063 |
device_type | Receiver |
family | |
latency | Seconds |
proteins | LuxR |
signalling_molecule | 3OC6HSL |
switch_point | 2nM |