Part:BBa_K515107
Composite p(tetR) - Dendra2
Description
Biobrick BBa_K515007, a translational subunit under the control of repressible promoter TetRBBa_R0040. Coding sequence BBa_K515007 codes for Dendra a photoconvertible reporter protein. In its natural state, it is excited and emits at 486 and 505nm, respectively. This appears green and therefore in its natural state Dendra can be used as GFP reporter. However it can be irreversibly converted to be excited at 558nm and consequently emit at 575nm by single-photon stimulation. After conversion reporter appears red and can be observed as RFP. Conversion can be efficiently achieved at wavelengths of both 488 and 405nm (Gurskaya et al., 2006). However, the cytotoxic wavelength of 405 converts Dendra more efficiently (data not shown).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterisation
Dendra can be efficiently converted using confocal microscopy.
| chassis | E. coli DH5α |
| control | R0040 |
| device_type | Fluorescent reporter |
| emission | 505 nm and 575 nm |
| excitation | 486 nm and 558 nm |
| protein | K515007 |
| rbs | B0034 |
| resistance | Chloramphenicol |