Translational_Unit

Part:BBa_K515000:Design

Designed by: Atipat Patharagulpong   Group: iGEM11_Imperial_College_London   (2011-09-01)
Revision as of 15:10, 12 September 2011 by Cs3109 (Talk | contribs)

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IaaM - tryptophan-2-mono-oxygenase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 450
    Illegal BamHI site found at 1395
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 157
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is an insulator sequence before the RBS. This sequence has been specially designed to contain no homology with the vector, to avoid recombination and easier PCR of the parts. The insulator also alows switching of the promoter without influencing the RBS.

The coding region has been optimised for B. subtilis and E. coli through the use of the program we have designed.

The coding region originates from P. savastanoi and has previously been expressed in E. coli [1]

Source

Genomics sequence originating from P. savastanoi, synthesized by Eurofins.

References

[1] Palm C. et al., 1988. Cotranscription of Genes Encoding Indoleacetic Acid Production in Pseudomonas syringae subsp. savastanoi. Journal of Bacteriology, 172(2), pp.1002-1009.