Part:BBa_K515100

IAA biosynthetic genes under control of the Pveg2 promoter

The IAM pathway is a two step pathway which generates indole-3-acetic acid (auxin) from the precursor tryptophan. IAA tryptophan monooxygenase (IaaM) BBa_K515000, catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which is hydrolyzed to indole-3-acetic acid and ammonia by indoleacetamide hydrolase (IaaH) BBa_K515001 . There are several different pathways that produce indole-3-acetic acid. IaaM and IaaH originate from P.savastanoi and have been expressed in E. coli previously, and shown to secrete auxin into cell supernatant, however without sufficient characterisation. [1]

Compatability

Chassis: This device has been tested in E. coli strain DH5α as a construct assembled with the pSB1C3 backbone vector.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 547
  Illegal BamHI site found at 1492
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 254
  Illegal NgoMIV site found at 2835
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1]Palm, CJ et al., 1989. Cotranscription of genes encoding indoleacetic acid production in Pseudomonas syringae subsp. savastanoi. Journal of Bacteriology, 171(2), pp.1002-1009.</p>