Device
eGFP-PTS1

Part:BBa_K678002

Designed by: DTU-Denmark-2   Group: iGEM11_DTU-Denmark-2   (2011-09-02)
Revision as of 12:00, 12 September 2011 by Jaide (Talk | contribs) (Usage and Biology)

eGFP-PTS1 for mammalian cells

Vector expressing eGFP from the CMV promoter with the BGH polyA tail and with a hygromycin resistance cassette targeted to the peroxisomes with the targeting sequence S-K-L often referred to as peroxisomal targeting sequence 1 (PTS1)

Usage and Biology

We used this device to target enhanced GFP to the peroxisomes of the mammalian cell line U-2 OS (See picture below). https://static.igem.org/mediawiki/igem.org/c/ce/26.08.2011_12-1%2B16_034.tif

This picture shows the mammalian cell line U-2 OS transfected with device BBa_K6788002 on a plasmid and a plasmid expressing Cyan fluorescent protein. https://static.igem.org/mediawiki/igem.org/6/67/26.08.2011_12-1%2B16_grøn_%2Bblå.tif https://static.igem.org/mediawiki/igem.org/6/67/26.08.2011_12-1%2B16_grøn_%2Bblå.tif File:Https://static.igem.org/mediawiki/igem.org/6/67/26.08.2011 12-1+16 grøn +blå.tif 26.08.2011_12-1+16_grøn_+blå.tif File:26.08.2011 12-1+16 grøn +blå.tif

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2233
    Illegal SpeI site found at 49
    Illegal PstI site found at 2323
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2233
    Illegal SpeI site found at 49
    Illegal PstI site found at 2323
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2233
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2233
    Illegal SpeI site found at 49
    Illegal PstI site found at 2323
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2233
    Illegal SpeI site found at 49
    Illegal PstI site found at 2323
    Illegal NgoMIV site found at 3088
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote
//chassis/eukaryote/human
//direction/forward
//function/reporter/fluorescence
//proteindomain/localization
Parameters
chassisMammalian cells
targetTargeting the peroxisomes