Part:BBa_K678002
eGFP-PTS1 for mammalian cells
Vector expressing eGFP from the CMV promoter with the BGH polyA tail and with a hygromycin resistance cassette targeted to the peroxisomes with the targeting sequence S-K-L often referred to as peroxisomal targeting sequence 1 (PTS1)
Usage and Biology
We used this device to target enhanced GFP to the peroxisomes of the mammalian cell line U-2 OS (See picture below). https://static.igem.org/mediawiki/igem.org/c/ce/26.08.2011_12-1%2B16_034.tif
This picture shows the mammalian cell line U-2 OS transfected with device BBa_K6788002 on a plasmid and a plasmid expressing Cyan fluorescent protein. https://static.igem.org/mediawiki/igem.org/6/67/26.08.2011_12-1%2B16_grøn_%2Bblå.tif https://static.igem.org/mediawiki/igem.org/6/67/26.08.2011_12-1%2B16_grøn_%2Bblå.tif File:Https://static.igem.org/mediawiki/igem.org/6/67/26.08.2011 12-1+16 grøn +blå.tif 26.08.2011_12-1+16_grøn_+blå.tif File:26.08.2011 12-1+16 grøn +blå.tif
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2233
Illegal SpeI site found at 49
Illegal PstI site found at 2323 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2233
Illegal SpeI site found at 49
Illegal PstI site found at 2323 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2233
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2233
Illegal SpeI site found at 49
Illegal PstI site found at 2323 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2233
Illegal SpeI site found at 49
Illegal PstI site found at 2323
Illegal NgoMIV site found at 3088 - 1000COMPATIBLE WITH RFC[1000]
//chassis/eukaryote/human
//direction/forward
//function/reporter/fluorescence
//proteindomain/localization
chassis | Mammalian cells |
target | Targeting the peroxisomes |