Coding
LigA
Part:BBa_K525730:Design
Designed by: Anna Drong Group: iGEM11_Bielefeld-Germany (2011-09-12)
DNA ligase from Escherichia coli (LigA) with PT7 and RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1449
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2047
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1663
Design Notes
PCR
Source
Escherichia coli