Coding
YFP:tetR

Part:BBa_K606025

Designed by: Kévin Yauy   Group: iGEM11_Paris_Bettencourt   (2011-07-27)
Revision as of 10:29, 12 September 2011 by Kyauy (Talk | contribs)

YFP:tetR recombinant fusion protein

YFP:tetR is a recombinant fusion protein. It is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO). Using the tetO array (BBa_K606026) composed by a 10kb tetO sequence, we can concentrate YFP:tetR in a few loci and increase the fluorescence sensibility.

Origins of YFP:tetR and tetO Array come from François-Xavier Barre, Andrew Wright and Dave Lane (Kinetics of plasmid segregation, Molecular Microbiology, 2004)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 631
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/reporter
Parameters
negative_regulators